Bradford quantification of protein concentration Brandford法测定蛋白浓度

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Standard Curve

mg of standard BSA	0.2 mg/mL BSA stock (mL)	dH2O (mL)	Bradford mix (mL)
1	5	795	200
2	10	790	200
4	20	780	200
6	30	770	200
8	40	760	200

Wait for 5 minutes and then read absorbance at 595nm

At the same time, prepare your samples of interest as above and meaure absorbance after measuring the standards. Take an average of the standard sample and determine the absorbance that defines 1 mg of BSA and use that to measure the concentration of the unknown samples.