PROTEASE
|
COMPANY
|
Protease Capture
|
Thrombin
Leu-Val-Pro-Arg▼Gly-Ser
|
Amersham-Biosciences, Novagen,
SIGMA, Roche
|
Benzamidine-Agarose
|
Factor Xa
Ile-Glu/Asp-Gly-Arg▼
|
Amersham-Biosciences, New England Biolabs,
Roche
|
Benzamidine-Agarose
|
Enterokinase
Asp-Asp-Asp-Asp-Lys▼
|
New England Biolabs,
Novagen, Roche
|
Trypsin Inhibitor-Agarose
|
TEV protease
Glu-Asn-Leu-Tyr-Phe-Gln▼Gly
|
Invitrogen – Life Technologies
|
Ni-NTA (6His recomb. TEV)
|
PreScission
Leu-Glu-Val-Leu-Phe-Gln▼Gly-Pro
|
Amersham-Biosciences
|
GSTrap for GST fusion enzyme
|
TAGZyme
His-tag removal by Exoproteolytic Digestion
|
Qiagen
|
Ni-NTA (6His recomb. enzyme)
|
Intein Site
dithiothreitol cleavage
|
New England Biolabs
|
DTT elimination by dialysis
|
HRV 3C Protease
Leu-Glu-Val-Leu-Phe-Gln▼Gly-Pro
|
Novagen
|
Ni-NTA (6His recomb. enzyme)
|
SUMO Protease
recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO
|
Invitrogen – Life Technologies, LifeSensors
|
Ni-NTA (6His recomb. enzyme)
|
蛋白质相互作用组学分析技