Methods in Molecular Biology VOLUME158

Gene Knockout Protocols 基因敲除实验方法

M. J. Tymms and I. Kola

Humana Press Inc.

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 PDF格式，共437页，4.5M，点击此处下载

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 内容：

1  Overview  Gene Knockouts

2  Isolation and Maintenance of Primate Embryonic Stem Cells

3  Gene Targeting in ES Cells

4  Manipulating Mouse Embryonic Stem Cells

5  Gene Targeting in a Centralized Facility

6  The LoxP/CRE System and Genome Modification

7  Creation and Use of a Cre Recombinase Transgenic Database

8  Choice of Mouse Strains for Gene Targeting

9  Isolation, Microinjection, and Transfer of Mouse Blastocysts

10 Aggregation Chimeras

11 How to Study Pathologic Phenotypes of Knockout Mice

12 Analysis of Hematopoietic Phenotypes in Knockout Mouse Models

13 Isolation of Embryonic Fibroblasts and Their Use in the In Vitro

    Characterization of Gene Function

14  Influence of Genetic Background on Knockout Mouse Phenotypes

15 Lineage Allocation During Early Embryogenesis

16 Generation of Double-Knockout Embryonic Stem Cells

17 In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes

18 Embryonic Stem Cells in the Study of Hematopoiesis

19 Interferon-Inducible ES Cell Expression Systems

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