|
MS Plant Tissue Culture Medium
| Component |
mg/l in MS |
mg/l in stock |
Amount for |
|
|
|
|
|
MS Macro I |
|
use as 10x stock |
1 liter |
| NH4NO3 |
1650 |
16.500 |
16.5 g |
| KNO3 |
1900 |
19.000 |
19.0 g |
| MgSO4.7H2O |
370.6 |
3706 |
3.706 g |
| KH2PO4 |
170 |
1700 |
|
| 100 ml/l |
|
|
|
|
|
|
|
| MS Macro II |
|
use as 10x stock |
1 liter |
| CaCl2.H2O |
439.8 |
4398 |
4.398 g |
|
(100 ml/l) |
|
|
|
|
|
|
|
|
FeNaEDTA |
|
use as 100x stock |
100 ml |
| 100 ml/l |
36.7 |
3670 |
367 g |
|
|
|
|
| MS Micros |
|
use as 1000x stock |
100 ml |
| H3BO3 |
6.20 |
6200 |
620 mg |
| MnSO4.2H2O |
13.2 |
13200 |
1320 mg |
| ZnSO4.7H2O |
8.6 |
8600 |
860 mg |
| Na2MoO4.2H2O |
0.25 |
250 |
25 mg |
| CuSO4.5H2O |
0.025 |
25 |
2.5 mg |
| CoCl2.2H2O |
0.025 |
25 |
2.5 mg |
| KI |
0.83 |
830 |
83 mg |
| 1 ml/l |
|
|
|
|
|
|
|
| Myo-inositol |
|
use as 100x stock |
100 ml |
|
100 |
10000 |
1 g |
| 10 ml/l |
|
|
|
|
|
|
|
| MS Vitamines |
|
use as 1000x stock |
100 ml |
| Nicotinic acid |
0.5 |
500 |
50 mg |
| Pyridoxine-HCl |
0.5 |
500 |
50 mg |
| Thiamine-HCl |
0.1 |
100 |
10 mg |
| Glycine |
2 |
2000 |
200 mg |
| 1 ml/l |
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|
Isolation of Chloroplast