Air dry flowers for 1-2 hours (optional--this helps with release from anthers but is not essential), then dab anthers onto surface of a 25-30µL drop of germination media. Drop can be on a depression slide or on a coated slide designed to keep samples separate. Use 1 flower per drop. Invert and incubate overnight in 100% humidity. Then place small drops of vacuum grease on the slide and gently place a coverslip on top. Allow the coverslip to just touch the drops of liquid but not to squish them. Hicks et al. Plant Phys 134: 1227-39. 2004