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| 发布时间：2007-01-14|
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Protocol for Sending Strains
You will need:
- Sterile pieces of foil (about 2.5 inches square). (Sterilize and store in a glass petri dish)
- 1/4" sterile disks (from Difco, #1599-35, concentration disks 1/4", sterile blanks)
- Remove a disk from the bottle using a sterile toothpick. place on a piece of sterile foil
- Wet disk with a drop of YEPD (yeast) or LB (bacteria).
- Pick a single colony from a plate with a toothpick and transfer to sterile disk
- Fold ends of foil to wrap disk, fold ends several times to prevent leaking
- Wrap with a piece of white tape and label with strain number
These can be taped to a sheet of paper with the genotype and mailed in a regular envelope
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Frozen Permanents