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FROGGING
- Frog is 8x6 prong. keep in EtOH, flame, then dip in sterile dH2O before use.
- Dip & swirl into yeast diluted in microtiter plates.
- Place on dry plate 1-2sec, remove, will leave 1-2 ul.
- Serial dilutions (in steril water on YPD) -8 samples x 6 dilutions. Aim for initial dilution at ~OD 0.1 [850 cells/uL] in 250 ul. 5X dilutions (do 4) 50 ul into 200 ul H2O
- Check plates, sometimes not even. Try pressing on FROG
| OD600 [cells/mL] |
cells/1 ul spot |
5X dilution |
| ~0.1 85x104 |
850 |
850 |
| 28x104 |
283 |
170 |
| 9.4 |
94 |
34 |
| 3.2 |
32 |
6.8 |
| 1 |
11 |
|
| 0.35 |
3.5 |
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Yeast sporulation (liqui