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| [ 文章来源： | 文章作者：
| 发布时间：2007-01-14|
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20X SSPE BUFFER
- 3 Liters
525.9g NaCl sodium chloride
82.8g NaH2PO4 sodium phosphate monobasic
28.2g EDTA powder FW=372
- bring up to 2400mls with H2O
- add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)
- autoclave for 20 min
- 2 Liters
350.6g NaCl sodium chloride
55.2g NaH2PO4 sodium phosphate monobasic
18.8g EDTA powder FW=372
- bring up to 1600mls with H2O
- add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)
- autoclave for 20 min
- 1 Liters
175.3g NaCl sodium chloride
27.6g NaH2PO4 sodium phosphate monobasic
9.4g EDTA powder FW=372
- bring up to 800mls with H2O
- add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)
- autoclave for 20 min
上一篇：10X TBE BUFFER 下一篇：0.5M EDTA
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10X TBE BUFFER