|
| |
|
|
| Removal of Counts : NaOH Method
|
| [ 文章来源： | 文章作者：
| 发布时间：2007-01-05|
字体：
[大
中
小]
| |
|
- Unwrap the membranes and stack them in a pile (Do not dry out the membranes completely).
- Place them individually into 1L 0.1 N NaOH 0.2 % SDS wash solution for exactly 20 min.
- Pour off the wash solution and neutralize with 1 L 0.5 M Tris (pH 7.5) 0.2 % SDS for 20 min.
- Place the membranes on 3 MM paper. Let dry completely between two sheets of 3MM paper and store in plastic bag at 4 C.
上一篇：Light Microscopy--光学显微镜 下一篇：Protocols: Acid Cleaning Coverslips
|
|
|
Light Microscopy--光学显