Laser Capture Microdissection (LCM)

• Introduction to LCM  (BJMU)
    
• Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections (NIH Laser Capture Microdissection Core Lab)
• Preparation and LCM of Paraffin Embedded Tissue Sections (NIH Laser Capture Microdissection Core Lab)
    
• DNA Extraction Protocol of LCM tissue and PCR for DNA analysis (NIH Laser Capture Microdissection Core Lab)
    
• RNA Extraction Protocol of LCM tissue and RT-PCR for mRNA analysis (NIH Laser Capture Microdissection Core Lab)
     
• Paraffin-Embedded Tissue for LCM (Arcturus)
  Paraffin embedding is a process in which fixed (formalin or other fixative) is infiltrated and then blocked out in paraffin to stabilize it for long-term storage and easy sectioning...
    
• H & E Staining Protocol for LCM (Arcturus)
    
• De-Paraffinization of Tissue Sections (Arcturus)
  Remove paraffin from section for tissue preparation such as nucleic acid extraction.
     
• DNA Extraction From Paraffin Section (Arcturus)
  This extraction method has been used for measuring loss of heterozygosity (LOH), dideoxy fingerprinting (DDF), clonality analysis (chromosome X inactivation) and direct sequencing of PCR* products for single base mutational analysis. Extractions are typically performed on 500-1000 captured cells.
    
• RNA Extraction from Paraffin Section (NIH Protocol) (Arcturus)
  A Protocol for extracting RNA from paraffin embedded tissue.
    
• cDNA Library Construction 
  Detailed protocol for cDNA library construction from microdissected material.