Making Antibody

·         Production of Polyclonal Antibody in Rabbit (Walter Steffen)
Provides detailed protocol for immunizatioin, bleeding procedure, obtaining serum, precipitation of IgG.

·         GST-fusion protein injection into rabbits (IVAN J. DELGADO ORLIC)

·         How Do I make an Antibody against a Peptides (Pierce)

·         A fast and inexpensive method for raising high-specificity polyclonal antibodies in mice (Technical Tips Online)

·         Guide to Antipeptide Antibody (Perkin-Elmer)
General Information about the design and synthesis of peptides for the production of anti-peptide antibodies, as well as an overview of the steps involved in the production of antibodies ­ immunization, collection of antisera, analysis of antisera, and antibody purification. Protocols for a number of these procedures are provided. 

o  Designing Antigens

o  Synthesizing Peptides

o  Conjugation of Peptides to Protein Carriers

o  Immunizations

o  Analysis of Antisera: Immunoassays

o  Purifying Antibodies 

• Preparation of Polyclonal Antibodies (PMCI)
  Immunization and antisera preparation
    
• Making antibodies to synthetic peptides (Daniel T. O'Connor)
    
• Preparation of Peptide-KLH Conjugates for Immunization (Mitshison Lab)
    
• Testing Rabbit Bleeds by Elisa (Mitshison Lab)
    
• Coupling Peptides to Resin for Affinity Purification (Mitshison Lab)
    
• Purification of Anti Peptide Antibody (Mitshison Lab)

Others

·         Tips and hints for the storage of antibodies (Synaptic Systems)