Antibody Purificatioin

• Purification of IgG Using Protein A- or Protein G-Agarose (KPL)
    
• Purifying Antibodies (Perkin-Elmer)

Precipitation Methods
Protein A or Protein G Columns or Beads
Antigen Columns

·         Affinity Purification of Antibodies from Crude Serum (PMCI)

·         Affinity Purification of Antibody (Walter Steffen)

• Coupling procedure
• Affinity purification
• Absorption of antibodies to acetone powder
• Production of acetone powder
• Extraction of antibodies from egg yolk
• Purification and cleavage of murine monoclonal antibodies
• IgG purification with protein G
• Digest of IgG with papain
    

·         Affinity Purification of Antibodies (Vesicle Trafficking)

·         Affinity Purification of Antibodies (XMMR)
protocol in great detail, including column preparation, wash and more...

·         Protein G Purification of Antibodies (Contributed by Nanci Donacki) 

·         Protein A Purification of Antibody (Contributed by Nanci Donacki) 

• Coupling Antibodies to Protein A/G (Breeden Lab)
    
• Coupling Peptides to Resin for Affinity Purification (Mitshison Lab)
    
• Purification of Anti Peptide Antibody (Mitshison Lab) 
      
• Antibody Purification (Mike A. Dyer) 
  This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography.

Column Preparation

• Making Affinity Columns (XMMR)