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| [ 文章来源： | 文章作者：
| 发布时间：2007-07-06|
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Liposome Preparation
OBJECTIVE:
Method for incorporating proteins into liposomes for liposome swelling assay or as an alternative antigen presentation method.
REAGENTS:
Dioleoyl Phosphatidyl Choline
Buffer of choice / distilled water
METHODS:
- Dry 0.5 mmole of dioleoyl phosphatidyl choline under nitrogen in a disposable glass tube.
- Evacuate in dessicator under vacuum for 30 minutes.
- Add buffer / dH20 to required volume and scrape the sides of the glass tube to dislodge the lipid.
- Add protein at 1 mg/ul of lipid used.
- Vortex for 30 seconds. Sonicate twice in a bath sonicator at 7 degree for 15 sec.
This makes multilamellar vesicles that become small unilamellar vesicles (SUV) with prolonged sonication time. To make large unilamellar vesicles, use the extruder.
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Labeled Sphingomyel