Immunization Protocol for Monoclonal Antibody Production

Antigens:

Proteins: 10-100 µg
Cells: 0.5 - 5.0 x10⁷

Adjuvant:

Complete Freunds Adjuvant
None

Schedule:

Day	Manipulation	Adjuvant	Site
0	Primary Immunization	CFA	s.c.
14	Boost #1	IFA	s.c.
24	Boost #2	IFA	s.c.
35	Boost #3	IFA	i.v.
42	Bleed and test for antibody
45	Boost #4	IFA	i.v.
66+	3-4 week rest followed by i.v. or i.p. boost	Saline/PBS	i.v.
69	fusion 3d after i.v. boost or 4d after i.p. boost	–	i.v.