注：这里的果蝇细胞系指的来源于国际果蝇基因组资源中心的

Drosophila Cell Lines in the DRSC（Drosophila Genomics Resource Center ）

Clone 8

Origin:

3rd instar larvae wing imaginal discs

Reference:

Peel et al., 1990RNAi_FAQ_lines.pl#note1">1

Received from:

Characteristics:

columnar epithelial, adherent, will form multiple layers, conserved signaling pathways

Karyotype:

Diploid

Drosophila Line 1 (otherwise known as Schneider Line 1) - (DL1)

Origin:

Dissociated embryos, near hatching (Oregon R)

Reference:

Schneider, 1972RNAi_FAQ_lines.pl#note2">2

Received from:

Ranjit Dasgupta-University of Wisconsin-Madison

Characteristics:

Hemocyte-like gene expression, phagocytic, adherent monolayer of uniformly round, smooth cells

Karyotype:

Drosophila Line 2 (otherwise known as Schneider Line 2) - (DL2)

Origin:

Dissociated embryos, near hatching (Oregon R)

Reference:

Schneider, 1972RNAi_FAQ_lines.pl#note2">2

Received from:

Peter Christian

Characteristics:

Hemocyte-like gene expression, phagocytic, adherent monolayer of uniformly round, smooth cells

Karyotype:

Kc (Kc 167)

Origin:

Dissociated embryos, 8 - 12 h (F2 ebony x sepia)

Reference:

Echalier and Ohanessian, 19693

Received from:

Cherbas

Characteristics:

Hemocyte-like gene expression, phagocytic, uniformly round, clump in sheets, ecdysone responsive into adherent, bipolar spindle-shaped cells

Karyotype:

up to 90% diploid, XX with one IVth punctiform chromosome

ML-DmBG2

Origin:

Dissociated 3rd instar larvae brain and ventral ganglia (y v f mal)

Reference:

Ui et al, 19944

Received from:

Characteristics:

acetylcholine, HRP expression, neuronal-like processes

Karyotype:

Diploid

Schneider's Line S2 - (S2)

Origin:

Dissociated embryos, near hatching (Oregon R)

Reference:

Schneider, 19722

Received from:

Invitrogen

Characteristics:

Hemocyte-like gene expression, phagocytic, semi-adherent in colonies, round, granular cytoplasm

Karyotype:

female, 60%-80% tetraploid

Schneider's Line S2-(SL2)

Origin:

Dissociated embryos, near hatching (Oregon R)

Reference:

Bruckner et al. 20005

Received from:

Steve Cohen

Characteristics:

Hemocyte-like gene expression, phagocytic, semi-adherent in colonies, round, granular cytoplasm.

Karyotype:

female, 60%-80% tetraploid

Schneider's Line S3

Origin:

Dissociated embryos, near hatching (Oregon R)

Reference:

Schneider, 19722

Received from:

Cherbas

Characteristics:

adherent, spindle-shaped cells, ecdysone responsive, grow in clumps

Karyotype:

XX and XY, 90% diploid

Schneider's S2 - (S2*)

Origin:

Dissociated embryos, near hatching (Oregon R)

Reference:

Schneider, 19722

Received from:

Maniatis

Characteristics:

Hemocyte-like gene expression, phagocytic, semi-adherent in colonies, round, granular cytoplasm

Karyotype:

Schneider's S2 - (S2-R+)

Origin:

Dissociated embryos, near hatching (Oregon R)

Reference:

Yanagawa, S. et al, 19986

Received from:

Yanagawa

Characteristics:

Hemocyte-like gene expression, phagocytic, adherent, flat cells; Fz+ and Wg-responsive

Karyotype:

Schneider's S2 - (S2C)

Origin:

Dissociated embryos, near hatching (Oregon R)

Reference:

Schneider, 19722

Received from:

Cherbas

Characteristics:

Hemocyte-like gene expression, semi-adherent in colonies, round, granular cytoplasm

Karyotype:

References:

1. Peel DJ, Johnson SA, Milner MJ. Tissue Cell. 1990;22(5):749-58.
2. Schneider I. J Embryol Exp Morphol. 1972 Apr;27(2):353-65.
3. Echalier, G. and A. Ohanessian. C R Acad Sci Hebd Seances Acad Sci D. 1969 Mar 31;268(13):1771-3.
4. Ui K, Nishihara S, Sakuma M, Togashi S, Ueda R, Miyata Y, Miyake T. In Vitro Cell Dev Biol Anim. 1994 Apr;30A(4):209-16.
5. Bruckner et al. 2000, Nature 406(6794):411-5
6. Yanagawa, S., J.S.Lee, A. Ishimoto. J Biol Chem. 1998 Nov 27;273(48)