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| CARBONATE TRANSFER SOLUTIONS
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| [ 文章来源： | 文章作者：
| 发布时间：2007-01-15|
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CARBONATE WESTERN TRANSFER SOLUTIONS
Dunn, Anal. Biochem. 1986: 157
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1.5L |
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1.0L
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| 10mM NaHCO3 |
1.26g |
chill to 4oC |
0.84g
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| 3mM Na2CO3 |
0.477g |
pH ~9.9 |
0.318g
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| in 20% MeOH |
300mL |
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200mL
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- Blot: no need to presoak gell.
Transblot: 1-2H @ 1A keep T<35oC
Miniblot: 1H @ 250mA
- Assemble wet sandwich on black side:
black side holder
brillo pad
3MM paper
gel
nitrocellulose
3MM
brillo
white side holder
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Western Blotting - Antib