Practical in situ Hybridization is aimed at researchers who wish to use efficient and reliable protocols in their work and those who need to check the validity and interpretation of published data. This book presents a reliable, tried-and-tested approach to the methodologies in current use in many laboratories.

 

 

DNA: DNA in situ hybridization PCR Labeling of cloned DNA sequences Ethanol Precipitation Checking Biotin and Digoxigenin labeled Probe Preparation of Chromosome Spreads Preparation of Acid-cleaned slides Accumulation & fixation of Plant metaphase chromosomes Squash preparation of plant chromosomes In situ Hybridization data sheet

Mitotic chromosome preparations from mammalian suspension cell cultures Quality control of chromosome preparations Pretreatment of chromosome preparations Hybridization mixture for cloned and genomic probes Denaturation and Hybridization Reprobing of preparations Strigent washes Fluorescent detection of hybridization sites Fluorescent staining of DNA and mounting Appendix - Buffers and other solutions