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| [ 文章来源： | 文章作者：
| 发布时间：2007-01-14|
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Preparation of gel for Posi blot transfer
- Cut gel to size of preexisting mask or cut new mask.
- For depurination: put gel in pyrex dish, add 500ml .25N HCl (10mL conc. HCl+450ml H2O) and shake 15min.
- Remove HCl, rinse w/H2O
- Add 500ml 1X Posiblot denaturing solution and shake 15min.
- Repaeat #4
- Remove denaturing solution
- Add 500ml Posi blot neutralizing solution and shake 15min.
- Repeat #7
- While gel is in 2nd neutralizing wash (step 8):
a) make up 1 liter 5XSSPE
b) cut gene screen plus membrane to size of gel and mark membrane w/pen
c) cut whatman larger than size of gel
d) soak gene screen plus and sponge in 5XSSPE
上一篇：ISOLATION OF HIGH MOLECULAR WEIGHT YEAST DNA 下一篇：RNA Isolation from Yeast
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Northern Blot--northern