Refolding of Denatured/Reduced Lysozyme Using Weak-Cation Exchange Chromatography
Yan WANG, Bo Lin GONG, Xin Du GENG， Chinese Chemical Letters, 14 (2003) 828 – 831
Oxidative refolding of the denatured/reduced lysozyme was investigated by using
weak-cation exchange chromatography (WCX). The stationary phase of WCX binds to the reduced lysozyme and prevented it from forming intermolecular aggregates. At the same time urea and ammonium sulfate were added to the mobile phase to increase the elution strength for lysozyme. Ammonium sulfate can more stabilize the native protein than a common eluting agent, sodium chloride. Refolding of lysozyme by using this WCX is successfully. It was simply carried out to obtain a completely and correctly refolding of the denatured lysozyme at high concentration of 20.0 mg/mL.
High-performance hydrophobic interaction chromatography as a tool for protein refolding
Xindu Geng and Xiaoqing Chang, J. Chromatogr., 599 (1992) 185-194
       A method for the refolding of previously unfolded proteins with a concentrated solution of denaturing agent is presented, involving the use of high-performance hydrophobic interaction chromatography (HPHIC) to separate the denaturing agent completely from the unfolded protein and to provide a suitable environment for its refolding. The retention, peak shape and peak height in HPHIC and size-exclusion chromatography, UV spectra, circular dichroic spectra and bioactivity were used to test the possibility and the completeness of the protein refolding. The proposed method permits the extracted solution from Escherichia coli cells to be injected directly into the HPHIC column and, at the same time, the refolding and purification of the proteins to be effected. The renaturation and purification of recombinant human interferon­‑g form E. coli cells is one example of the application of the method in biotechnology.
疏水色谱法的进展及其在生化研究中的应用
刘彤, 耿信笃, 色谱, 1998, 16 (1) : 30-34
着重评述了疏水色谱法的理论研究及疏水固定相中无机填料、有机填料、非多孔填料以及大孔膜的新发展，并对疏水色谱法在生物大分子的分离、纯化和生化研究中的应用,包括在蛋白质复性、折叠和分子构象变化等方面的应用作了介绍，全文包括62篇文献和一张表格。
多功能蛋白增活器的研制及性能研究
刘彤, 耿信笃, 西北大学学报(自然科学版), 1999, 29 (2) : 123-126
自制了一种新型的多功能蛋白增活器，它具有同时除变性剂、分离杂蛋白、复性目标蛋白及便于回收变性剂的功能。考查了其对蛋白的分离、复性性能。发现这种多功能蛋白增活器可对6种标准蛋白进行良好地分离。同时对盐酸胍变性的溶菌酶和核糖核酸酶完全复性。通过测定压力-流速曲线，发现多功能蛋白增活器的压力均远远低于普通色谱柱。将其应用于基因工程发酵的粒细胞集落因子(G-CSF)的分离纯化，可使纯度接近于100%，生物活性大于常规方法3倍。
蛋白的色谱复性及同时纯化
郭立安, 耿信笃, 生物工程学报, 2000, 16 (6) : 661-666
对近年来新发展的用液相色谱(ＬＣ)进行蛋白质复性及同时纯化的方法做了评述，详细介绍了蛋白质在4种液相色谱上的复性及同时纯化的方法、设备和影响因素，并对各自的优缺点进行了比较，为色谱法作为研究蛋白质折叠及用于基因工程生产治疗蛋白质的复性及同时纯化技术的进一步应用提供依据。