Brief Guidline on

Diversity Database Fringerprinting Software (version 2, Bio-Rad)

based on Guidline Book and experience

Database file: Open file/a new database /savings (to invert data)

Edit lane:

Lane/Auto frame/manual framlane

Background subtraction:Lane/lane background/ (Raw & Bkg; Automatic; Rolling Disk; Disk size:usully 50-150, but my last time selecting 10)/Apply/tick gel.

Edit Band:

Automatically identifying

Manual identifying and editing individual bands by examing magnified images and absorption peaks using create, adjust and remove bands buttons

# Band attribution: Band/band attraibution /try bracket or line (firstly identify bands in the bracket condition, then adjust bands and export in the line condition).

   After identifiying, renumber the bands by edit manu/sorted and recalculate.

Buiding a Database:

Creating a Band set: Database/band set/ New or known.

For New: new/units/Molecular weight or Normalized Rf if not Molecular weight/select/Ok/match/click lane that you want to use as band set/proceeding with matching/tolerance (<2.5: for closing spaced band or >3.5: for widely spaced band)/ yes/green colour: indicating band set; red: match; yellow: unmatch band.

For known band set: band set/known band set name/applying lane

Edit yellow band:click match button/ then click it into new band type.

Assign a particular band type to a band on your image: Band set/ select your band set name/click on the numbered arrow button in the type column to assign band type to the mouse, the click on the band on your image (book 8-10)

Database Organization:

Organizing gel and samples (lane); selecting expected lane in database summary form

Database search and export results:

Open database summary form/click Gels button in database summary form/click “►”beside gel name/click “show layout”/set up background subtract as on, and disk size (For Feeding effects samples: 120)/click samples button/input expected date, name or category and click select button to select by date or name or category (in my analysis, by date)/delete no necessary lanes/close sample form/ search button (selecting method Dic coefficient; Ref lane: firstly click this button then click Ref lane; samilirity:last time 0.0%, selecting same data or category with that in sample form)/search/population form will be opened/ export results by click related button/print by clicking printer Fig or export and save in file by clicking “田” Fig.

*but save phylogenetic tree in file: open “phylogenetic tree” window, then click “Alt/Print” for copy this window, then open new word window, click “Ctrl/V” to paste the file to new word window or any place expected, the click the function colume in the right. Open function button “女“ click side line to omit no used parts, finally save.

# adding gel into database:

Open gel image added and saving in 1sc file/ open database summary form/click Gels button in database summary form/click Add gel/click gel image added (opened above); then Edit lane/Edit Band/Band set apply: band set/know band set name/apply as described in book/click gel/ Assign a particular band type to a band/ edit yellow bands/ renumber the bands by edit manu /sorted and recalculate/save

Recover lanes (samples) deled or Chang name of lanes (samples)

Open database summary form/click Gels button in database summary form/click “►”beside gel name/click “show layout”/re-adding original samples names to recover samples deled or changing sample names under sample column, then close.