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| What is the highest temperature
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| [ 文章来源： | 文章作者：
| 发布时间：2006-09-24|
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Question | | |
What is the highest temperature that SUPERSCRIPT II, MMLV, or THERMOSCRIPT can be used? |  | | |
Answer | | |
For RT-PCR, SUPERSCRIPT II RT can be used up to 50 C. MMLV can be used at temperatures up to 42 C. THERMOSCRIPT can be used at temperatures as high as 70 C (especially for amplicons expected to be 1 kb or less). For PCR products expected to be greater than 1 kb a maximum first strand synthesis temperature of 65oC is suggested. Be sure your first-strand primer anneals at the high temperature. |
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