PCR and multiplex PCR guide

Topics for PCR optimization
0. Standards: examples of multiplex reactions used in all experiments
1	Generalities Components, PCR cycle, vials	9	Annealing time and temperature
2	Choosing PCR primers How to design the primers	10	Extension time and temperature
3	Reaction volumes Do they influence the results?	11	DNA template concentration
4	Number of PCR products Multiplexing. How ?	12	Taq polymerase(s) Comparisons and concentration
5	Primer amount How much primer?	13	dNTP concentration Interaction with magnesium
6	PCR buffers Comparisons and concentration	14	MgCl2 concentration Interaction with dNTP and salt (PCR buffer)
7	Salt (KCl) concentration Essential optimization factor	15	Gel electrophoresis Agarose and polyacrylamide (PAA)
8	Designing PCR programs Customize them for your purpose	16	Adjuvants in PCR DMSO, glycerol and BSA
Some applications of PCR and multiplex PCR
A	Microdeletion screening	D	PCR labeling
B	LOH	E	InterAlu-PCR
C	Quantitative multiplex PCR*	F	Degenerative priming PCR