Assay Design for MIP Genotyping

Description:
For each SNP a single MIP probe is designed. A minimum of 5000 probes are designed as a probe set.

1) MIP probe design

Two homologies are designed that abut, but do not overlap the SNP. The tag sequence, two primer
sequences and a restriction sequence are added to homology 1 and homology 2 in the order (5' - 3')
homology 2, tag sequence, restriction site, primer P2, primer P1, and homology 1.