Lab of Dr. Mark Barton Frank, Oklahoma Medical Research Foundation
 
Please read the excellent descriptions of handling RNA and of cDNA synthesis by J. Sambrook, E. F. Fritsch, and T.Maniatis in Molecular Cloning, A Laboratory Manual (Cold Spring Harbor Laboratory Press, Second Edition, New York, 1989). The methodology for the synthesis of the first strand of the cDNA summarized below is based primarily on this source.…

http://omrf.ouhsc.edu/~frank/CDNA.html