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| DNA FRAGMENT PURIFICATION W/ GLASS WOOL
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| [ 文章来源： | 文章作者：
| 发布时间：2007-01-14|
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I. Glass wool method:
- Run TAE agarose gel and cut the appropriate band out with a clean razor blade.
- Poke a small hole with hot needle on the bottom of an eppendorf tube, and jam the hole with a little of siliconized glass wool.
- Put the gel in the tube and put this tube onto another tube and spin with 5-7 Krpm for 5-10 min. Add 1/2 vol. of phenol and 1/10 vol. of 3 M NaAc of the gel volume to the agarose tube and spin for another 5 min.
- Discard the agarose tube. Add 1/2 vol. of chloroform to the liquid and spin at 14 k
for 5'. Take the top layer and precipitate with 2-2.5 vol. of ethanol for 30 '. Spin and wash with 70% ethanol.
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