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| DNA Purification from Gels
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| [ 文章来源： | 文章作者：
| 发布时间：2006-09-26|
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1. Remove gel slice contain DNA fragment and place in 10 volumes of:
300 mM NaOAc, pH 7.0 300 ml 1 M NaOAC, pH 7.0
1 mM EDTA 2 ml 500 mM EDTA, pH 8.0
698 ml ddH20
2. Incubate at 22 C for 30 min. Transfer gel slice to a fresh tube.
3. Place tube in a Dry Ice/Ethanol bath for 5 min.
4. Puncture the bottom of a 0.5 mL microcentrifuge tube with a needle. Place the gel slice into this tube. Place this tube inside a 1.5 mL microcentrifuge tube.
5. Centrifuge for 15 min.
6. Collect the Eluent from the 1.5 mL eppendorf tube. Extract and precipitate the DNA.
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