|
- macerate tissue in Eppendorf tube without butter at RT
- add 400 ml extraction buffer
- vortex for 4 sec
- leave sample at RT until other samples are ready (> 1 h)
- spin in microfuge for 1 min
- transfer 300 ml of supernatant to different Eppendorf tube (prefilled with 300 ml isopropanole)
- mix and leave at RT for 2 min
- spin for 5 min
- vacuum dry pellet and take up in 100 ml TE
- use 1-2.5 ml for PCR
Remarks:
DNA is stable for one year at 4°C
| |
Solutions: |
| |
|
Extraction buffer: 200 mM Tris-HCl pH 7.5
250 mM NaCl
25 mM EDTA
0.5% SDS | |
ES CELL DNA&nb